![]() Rincon-Restrepo, M., Mikhailova, E., Bayley, H. Replication of individual DNA molecules under electronic control using a protein nanopore. Proof-of-principle experiments demonstrating that a biological nanopore with a coupled polymerase can be used for both strand sequencing and mechanistic studies of enzyme function. ![]() ![]() Processive replication of single DNA molecules in a nanopore catalyzed by phi29 DNA polymerase. A single-molecule nanopore device detects DNA polymerase activity with single-nucleotide resolution. Sequence-specific detection of individual DNA polymerase complexes in real time using a nanopore. Single molecule measurements of DNA transport through a nanopore. Microsecond time-scale discrimination among polycytidylic acid, polyadenylic acid, and polyuridylic acid as homopolymers or as segments within single RNA molecules. This is the first experimental report of electronic detection of a DNA molecule passing through a nanopore (α-haemolysin) and marks the start of the nanopore sequencing field.Īkeson, M., Branton, D., Kasianowicz, J. Characterization of individual polynucleotide molecules using a membrane channel. Nanopores: Sensing and Fundamental Biological Interactions (Springer, 2011). This review article provides a historical perspective on the field of nanopore DNA sequencing and elaborates on nanopore-based enzyme-mediated sequencing approaches. Nanopore analysis of nucleic acids bound to exonucleases and polymerases. This review article assesses the feasibility of various nanopore sequencing techniques that are currently under development (both optical and electrical).ĭeamer, D. The potential and challenges of nanopore sequencing. Nanopore-based single-molecule DNA analysis. Nanopore sequencing technology: research trends and applications. Characterization of nucleic acids by nanopore analysis. Characterization of individual polymer molecules based on monomer-interface interactions. Means for counting particles suspended in a fluid. Sequencing technologies - the next generation. mendelian disease: Evolutionary evidence for differences in molecular effects. Coding single-nucleotide polymorphisms associated with complex vs.
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